Hotspot

trnT-UGU-trnL-UAA

LSC · 45084-45938 · 855 bp

Back to Stemona

Pi 0.00955

MarkerSeek score 55.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 18
pi: 0.00955
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CTAACCTCTGAGCTAAGCGG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.5	58.0	894-950 bp	1.0	79.4
trnT-UGU-trnL-UAA_p2	CTAACCTCTGAGCTAAGCGG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.5	58.1	902-958 bp	1.0	78.8
trnT-UGU-trnL-UAA_p3	CTAACCTCTGAGCTAAGCGG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.5	57.8	917-973 bp	1.0	77.5
trnT-UGU-trnL-UAA_p4	CTAACCTCTGAGCTAAGCGG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.5	58.2	908-964 bp	1.0	77.4
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.5	57.7	914-970 bp	1.0	77.1