Hotspot

trnE-UUC-trnT-GGU

LSC · 29949-30542 · 594 bp

Back to Stemona

Pi 0.0125

MarkerSeek score 64.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 594
variable_sites: 17
pi: 0.0125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	670-697 bp	1.0	83.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	649-676 bp	1.0	83.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	635-662 bp	1.0	83.1
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	614-641 bp	1.0	83.1
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	667-694 bp	1.0	82.7