Hotspot

rpoB-trnC-GCA

LSC · 25461-26680 · 1220 bp

Back to Stemona

Pi 0.00862

MarkerSeek score 60.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 25
pi: 0.00862
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1354-1387 bp	1.0	77.1
rpoB-trnC-GCA_p2	TTGTGTACATTCCATCCCGC	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.3	57.5	1284-1317 bp	1.0	76.5
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1391-1424 bp	1.0	75.1
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTG	GACACCCGGATTTGAACTGG	50.0	55.0	58.2	58.8	1377-1410 bp	1.0	74.5
rpoB-trnC-GCA_p5	TTGTGTACATTCCATCCCGC	TTTGTTGATCAGGCGACACC	50.0	50.0	58.3	58.8	1321-1354 bp	1.0	74.5