Hotspot

rps16-trnQ-UUG

LSC · 5721-6241 · 521 bp

Back to Stemona

Pi 0.0111

MarkerSeek score 61.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 13
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	605-615 bp	1.0	84.1
rps16-trnQ-UUG_p2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	603-613 bp	1.0	84.1
rps16-trnQ-UUG_p3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	611-621 bp	1.0	84.0
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	587-597 bp	1.0	83.4
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	598-608 bp	1.0	82.8