Hotspot

petB

LSC · 77124-78582 · 1459 bp

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Pi 0.00137

MarkerSeek score 49.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1459
variable_sites: 2
pi: 0.00137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GCAGTTCGACCGCAAAATTT	TTCCTCCTCCAACTAATAAGA	45.0	38.1	58.2	53.0	1576-1577 bp	1.0	40.1
petB_p2	GCAGTTCGACCGCAAAATTT	TCCTCCTCCAACTAATAAGAT	45.0	38.1	58.2	52.7	1575-1576 bp	1.0	40.1
petB_p3	GCAGTTCGACCGCAAAATTT	ATTCCTCCTCCAACTAATAAGA	45.0	36.4	58.2	53.7	1577-1578 bp	1.0	40.1
petB_p4	GCAGTTCGACCGCAAAATTT	TTCCTCCTCCAACTAATAAGAT	45.0	36.4	58.2	53.7	1576-1577 bp	1.0	40.1
petB_p5	GCAGTTCGACCGCAAAATTT	ATTCCTCCTCCAACTAATAAGAT	45.0	34.8	58.2	54.3	1577-1578 bp	1.0	40.1