Hotspot

accD

LSC · 60215-61789 · 1575 bp

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Pi 0.0019

MarkerSeek score 59.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1575
variable_sites: 3
pi: 0.0019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCATGTAAATAGAGGCAAGCTCT	ACCCCATTAGTCCGACTTTG	39.1	50.0	57.4	57.2	1670 bp	1.0	44.9
accD_p2	TCTGGTGATTGTGTCTATATGCT	ACCCCATTAGTCCGACTTTG	39.1	50.0	57.1	57.2	1758 bp	1.0	42.9
accD_p3	TCATGTAAATAGAGGCAAGCTCT	AACCCCATTAGTCCGACTTTG	39.1	47.6	57.4	57.9	1671 bp	1.0	42.6
accD_p4	TCATGTAAATAGAGGCAAGCTCT	CCCCATTAGTCCGACTTTGA	39.1	50.0	57.4	56.9	1669 bp	1.0	42.6
accD_p5	TTCATGTAAATAGAGGCAAGCTCT	ACCCCATTAGTCCGACTTTG	37.5	50.0	58.0	57.2	1671 bp	1.0	41.8