Hotspot

ndhF-rpl32

SSC · 130854-131302 · 449 bp

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Pi 0.0204

MarkerSeek score 69.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 449
variable_sites: 9
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGGAAAAAGCAGAAGTCCT	AAAAAGCTTTCAAGGCTGCC	45.0	45.0	57.1	57.8	632-637 bp	1.0	72.7
ndhF-rpl32_p2	GTCGGAAAAAGCAGAAGTCC	AAAAAGCTTTCAAGGCTGCC	50.0	45.0	57.0	57.8	633-638 bp	1.0	72.4
ndhF-rpl32_p3	AGGTTGTTTGTTGATGTCGGA	AAAAAGCTTTCAAGGCTGCC	42.9	45.0	58.1	57.8	648-653 bp	1.0	71.5
ndhF-rpl32_p4	GGTTGTTTGTTGATGTCGGA	AAAAAGCTTTCAAGGCTGCC	45.0	45.0	56.6	57.8	647-652 bp	1.0	68.7
ndhF-rpl32_p5	AGGTTGTTTGTTGATGTCGG	AAAAAGCTTTCAAGGCTGCC	45.0	45.0	56.6	57.8	648-653 bp	1.0	68.6