Hotspot

trnL-UAG-ndhF

IRb · 127627-129483 · 1857 bp

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Pi 0.00324

MarkerSeek score 50.20

Primer available yes

Label trnL-UAG-ndhF

Feature Metadata

label_name: trnL-UAG-ndhF
feature_type: igs
region: IRb
length_bp: 1857
variable_sites: 6
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ndhF_p1	TTATAAGACGCTATGCCGCC	CTTGGTTCACGGCTGGATTA	50.0	50.0	57.9	57.9	2006-2011 bp	1.0	79.0
trnL-UAG-ndhF_p2	TCCTAAGAGCAGCGTGTCTA	CTTGGTTCACGGCTGGATTA	50.0	50.0	57.9	57.9	1952-1957 bp	1.0	78.6
trnL-UAG-ndhF_p3	TTATAAGACGCTATGCCGCC	TGGTTCACGGCTGGATTAAC	50.0	50.0	57.9	58.2	2004-2009 bp	1.0	78.4
trnL-UAG-ndhF_p4	AGCAGCGTGTCTACCAATTT	CTTGGTTCACGGCTGGATTA	45.0	50.0	57.8	57.9	1945-1950 bp	1.0	78.1
trnL-UAG-ndhF_p5	CTAAGAGCAGCGTGTCTACC	CTTGGTTCACGGCTGGATTA	55.0	50.0	57.8	57.9	1950-1955 bp	1.0	78.0