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psbE-petL

LSC · 67670-69100 · 1431 bp

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Pi 0.0028

MarkerSeek score 60.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1431
variable_sites: 4
pi: 0.0028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGAGGTTATAGTTAAAGCCA	50.0	39.1	57.9	57.3	1595-1597 bp	1.0	50.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGTCGTATCTTACTCAGACCA	50.0	42.9	57.9	55.3	1624-1626 bp	1.0	50.1
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GCTGAGGTTATAGTTAAAGCCAC	50.0	43.5	57.9	57.3	1594-1596 bp	1.0	49.9
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGAGGTTATAGTTAAAGCCA	50.0	39.1	57.8	57.3	1518-1520 bp	1.0	49.4
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCTGAGGTTATAGTTAAAGCCA	45.0	39.1	58.2	57.3	1604-1606 bp	1.0	49.3