Hotspot

ycf3-trnS-GGA

LSC · 47295-48058 · 764 bp

Back to Stellera

Pi 0.00262

MarkerSeek score 60.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 2
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	TGAATTAGGTGAAGGTGCGG	47.6	50.0	58.0	57.9	890-892 bp	1.0	71.0
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTAGGTGAAGGTGCGGAAAG	47.6	50.0	58.0	57.8	886-888 bp	1.0	70.4
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	824-826 bp	1.0	70.3
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	GTGCGGAAAGAGAGGGATTC	47.6	55.0	58.0	58.3	876-878 bp	1.0	69.1
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	GAATTAGGTGAAGGTGCGGA	47.6	50.0	58.0	57.6	889-891 bp	1.0	68.7