Hotspot

trnE-UUC-trnT-GGU

LSC · 33093-33751 · 659 bp

Back to Stellera

Pi 0.00607

MarkerSeek score 62.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 4
pi: 0.00607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	800 bp	1.0	79.1
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	779 bp	1.0	78.6
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	776 bp	1.0	78.4
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	762 bp	1.0	78.3
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	797 bp	1.0	77.9