Hotspot

trnS-GCU-trnG-UCC

LSC · 8982-9714 · 733 bp

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Pi 0.00274

MarkerSeek score 60.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 2
pi: 0.00274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	845-846 bp	1.0	72.7
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	844-845 bp	1.0	72.7
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	844-845 bp	1.0	72.7
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	843-844 bp	1.0	72.7
trnS-GCU-trnG-UCC_p5	CGCACAACGGATTAGCAATC	ATCAAACCGAAAGACCCCTT	50.0	45.0	57.9	57.0	859-860 bp	1.0	72.0