Hotspot

rps16-trnQ-UUG

LSC · 6314-7640 · 1327 bp

Back to Stellera

Pi 0.00234

MarkerSeek score 63.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1327
variable_sites: 3
pi: 0.00234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATCAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1415-1458 bp	1.0	81.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.1	58.0	1374-1417 bp	1.0	81.4
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1426-1469 bp	1.0	80.9
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	TTTGGTCCCGCTATTCGAAG	50.0	50.0	58.2	58.0	1385-1428 bp	1.0	80.9
rps16-trnQ-UUG_p5	CCACATCGCTTCAAACGAAG	GAAATCAAAATGGGGCGTGG	50.0	50.0	57.8	58.0	1395-1438 bp	1.0	80.7