Hotspot

trnL-UAG-ccsA

SSC · 109892-109999 · 108 bp

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Pi 0.0498

MarkerSeek score 66.40

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 108
variable_sites: 10
pi: 0.0498
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGAGTCAAAATATGTTCTAAAGTCGA	50.0	30.8	57.9	56.9	181-210 bp	1.0	48.0
trnL-UAG-ccsA_p2	GGTAGACACGCTGCTCTTAG	TGAGTCAAAATATGTTCTAAAGTCGA	55.0	30.8	57.8	56.9	183-212 bp	1.0	48.0
trnL-UAG-ccsA_p3	GTAGACACGCTGCTCTTAGG	TGAGTCAAAATATGTTCTAAAGTCGA	55.0	30.8	57.8	56.9	182-211 bp	1.0	48.0
trnL-UAG-ccsA_p4	AAATTGGTAGACACGCTGCT	TGAGTCAAAATATGTTCTAAAGTCGA	45.0	30.8	57.8	56.9	188-217 bp	1.0	47.9
trnL-UAG-ccsA_p5	AATTGGTAGACACGCTGCTC	TGAGTCAAAATATGTTCTAAAGTCGA	50.0	30.8	58.3	56.9	187-216 bp	1.0	47.9