Hotspot

trnM-CAU-atpE

LSC · 49577-49792 · 216 bp

Back to Stellaria

Pi 0.0496

MarkerSeek score 66.90

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 22
pi: 0.0496
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TTGACGTAGGTCTGCCTTTG	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.8	58.0	911-952 bp	1.0	83.5
trnM-CAU-atpE_p2	TTGACGTAGGTCTGCCTTTG	TGCTGAGAAGGGTAGTGACA	50.0	50.0	57.8	58.0	1024-1065 bp	1.0	83.2
trnM-CAU-atpE_p3	TTGACGTAGGTCTGCCTTTG	TACGCCTTAACGACCAATGG	50.0	50.0	57.8	58.0	1112-1153 bp	1.0	82.9
trnM-CAU-atpE_p4	TTGACGTAGGTCTGCCTTTG	CCCTATTGCCACAGCTGTAG	50.0	55.0	57.8	58.0	1150-1191 bp	1.0	82.7
trnM-CAU-atpE_p5	TTGACGTAGGTCTGCCTTTG	AAGAGCGCGAAAAATTGAGC	50.0	45.0	57.8	58.0	1491-1532 bp	1.0	81.9