Hotspot

ndhC-trnV-UAC

LSC · 48290-48670 · 381 bp

Back to Stellaria

Pi 0.0373
MarkerSeek score 61.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
381
variable_sites
29
pi
0.0373
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATGTCATATTCA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.0 57.8 446-866 bp 1.0 53.3
ndhC-trnV-UAC_p2 AAAATGCCCAGAAAATGTCA AGAAGGTCTACGGTTCGAGT 35.0 50.0 53.9 57.8 450-870 bp 1.0 52.3
ndhC-trnV-UAC_p3 AAAAATGCCCAGAAAATGTCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 54.8 57.8 451-871 bp 1.0 51.3
ndhC-trnV-UAC_p4 TGCCCAGAAAATGTCATATTCA GAAGGTCTACGGTTCGAGTC 36.4 55.0 56.0 57.5 445-865 bp 1.0 50.5
ndhC-trnV-UAC_p5 AAATGCCCAGAAAATGTCAT AGAAGGTCTACGGTTCGAGT 35.0 50.0 53.7 57.8 449-869 bp 1.0 50.3