Hotspot

ndhK-ndhC

LSC · 47886-47926 · 41 bp

Back to Stellaria

Pi 0.0505

MarkerSeek score 66.50

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 41
variable_sites: 7
pi: 0.0505
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GTGGAAACTCAATGGAATTCA	CATGGCGAAAAGGAGCATTG	38.1	50.0	54.4	58.1	112-114 bp	1.0	47.9
ndhK-ndhC_p2	GTGGAAACTCAATGGAATTCA	GCGAAAAGGAGCATTGGAAT	38.1	45.0	54.4	57.1	108-110 bp	1.0	45.9
ndhK-ndhC_p3	GTGGAAACTCAATGGAATTCAT	CATGGCGAAAAGGAGCATTG	36.4	50.0	55.0	58.1	112-114 bp	1.0	45.7
ndhK-ndhC_p4	TGGAAACTCAATGGAATTCA	CATGGCGAAAAGGAGCATTG	35.0	50.0	52.6	58.1	111-113 bp	1.0	45.7
ndhK-ndhC_p5	GTGGAAACTCAATGGAATTCA	TATGCATGGCGAAAAGGAGC	38.1	50.0	54.4	59.0	116-118 bp	1.0	45.5