Hotspot

psaA-ycf3

LSC · 39835-40877 · 1043 bp

Back to Stellaria

Pi 0.0294

MarkerSeek score 59.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1043
variable_sites: 61
pi: 0.0294
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ACGACGTTTTTACGGGATCC	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.3	57.8	836-1144 bp	1.0	85.1
psaA-ycf3_p2	GACGTTTTTACGGGATCCCT	TTGGTTGAAGATCACGGGAC	50.0	50.0	57.6	57.8	834-1142 bp	1.0	84.3
psaA-ycf3_p3	CCCATTCCTCAAACGACGTT	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.5	57.8	848-1156 bp	1.0	83.5
psaA-ycf3_p4	TTTTGACTTCTGGTTCCGGC	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.7	57.8	803-1111 bp	1.0	82.1
psaA-ycf3_p5	CGACGTTTTTACGGGATCCC	TTGGTTGAAGATCACGGGAC	55.0	50.0	59.0	57.8	835-1143 bp	1.0	79.4