Hotspot

ndhE-ndhG

SSC · 120545-120705 · 161 bp

Back to Stelis

Pi 0.0432
MarkerSeek score 74.20
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
161
variable_sites
12
pi
0.0432
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 AGTGCTCTAACCATGCTTCG AGGTGCAATTACTATGGCTCG 50.0 47.6 58.0 58.2 270-281 bp 1.0 78.1
ndhE-ndhG_p2 CTCTAACCATGCTTCGGCTT AGGTGCAATTACTATGGCTCG 50.0 47.6 58.0 58.2 266-277 bp 1.0 78.0
ndhE-ndhG_p3 AGTGCTCTAACCATGCTTCG GGTGCAATTACTATGGCTCG 50.0 50.0 58.0 56.7 269-280 bp 1.0 76.8
ndhE-ndhG_p4 CTCTAACCATGCTTCGGCTT GGTGCAATTACTATGGCTCG 50.0 50.0 58.0 56.7 265-276 bp 1.0 76.7
ndhE-ndhG_p5 TCTAACCATGCTTCGGCTTG AGGTGCAATTACTATGGCTCG 50.0 47.6 58.3 58.2 265-276 bp 1.0 76.2