Hotspot

ccsA-ndhD

SSC · 117558-117834 · 277 bp

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Pi 0.0499
MarkerSeek score 80.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
277
variable_sites
26
pi
0.0499
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACGAATTCGGCATTTGTAGC CTCGTTATCGGTTGACAAGGT 45.0 47.6 57.2 58.0 455-462 bp 1.0 70.6
ccsA-ndhD_p2 ACGAATTCGGCATTTGTAGC TCGTTATCGGTTGACAAGGT 45.0 45.0 57.2 56.9 454-461 bp 1.0 70.1
ccsA-ndhD_p3 ACGAATTCGGCATTTGTAGC TCTCGTTATCGGTTGACAAGG 45.0 47.6 57.2 57.8 456-463 bp 1.0 69.2
ccsA-ndhD_p4 ACGAATTCGGCATTTGTAGC TCGGTTGACAAGGTACAAGT 45.0 45.0 57.2 56.7 448-455 bp 1.0 68.8
ccsA-ndhD_p5 CGAATTCGGCATTTGTAGCT CTCGTTATCGGTTGACAAGGT 45.0 47.6 56.9 58.0 454-461 bp 1.0 68.3