Hotspot

trnL(uag)-ccsA

SSC · 116433-116570 · 138 bp

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Pi 0.0531
MarkerSeek score 74.50
Primer available yes
Label trnL(uag)-ccsA

Feature Metadata

label_name
trnL(uag)-ccsA
feature_type
igs
region
SSC
length_bp
138
variable_sites
13
pi
0.0531
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(uag)-ccsA_p1 TAGACACGCTGCTCTTAGGA GTCCCGAAGAAATCCAACGA 50.0 50.0 57.9 57.9 389-392 bp 1.0 82.2
trnL(uag)-ccsA_p2 ATACCCCGACTGAGAACGAA ATCCGCATAAAAGAGCTGCA 50.0 45.0 58.2 58.0 923-931 bp 1.0 81.9
trnL(uag)-ccsA_p3 ATACCCCGACTGAGAACGAA TCCGCATAAAAGAGCTGCAT 50.0 45.0 58.2 58.0 922-930 bp 1.0 81.9
trnL(uag)-ccsA_p4 TAGACACGCTGCTCTTAGGA ATCCGCATAAAAGAGCTGCA 50.0 45.0 57.9 58.0 656-659 bp 1.0 81.6
trnL(uag)-ccsA_p5 TAGACACGCTGCTCTTAGGA TCCGCATAAAAGAGCTGCAT 50.0 45.0 57.9 58.0 655-658 bp 1.0 81.6