Hotspot

rpl32-trnL(uag)

SSC · 115551-116352 · 802 bp

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Pi 0.0317
MarkerSeek score 75.30
Primer available yes
Label rpl32-trnL(uag)

Feature Metadata

label_name
rpl32-trnL(uag)
feature_type
igs
region
SSC
length_bp
802
variable_sites
48
pi
0.0317
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL(uag)_p1 TCCACCGGACAGTCAAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 881-901 bp 1.0 85.7
rpl32-trnL(uag)_p2 TCCACCGGACAGTCAAAAAG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 874-894 bp 1.0 85.3
rpl32-trnL(uag)_p3 TCCACCGGACAGTCAAAAAG CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 879-899 bp 1.0 85.2
rpl32-trnL(uag)_p4 TCCACCGGACAGTCAAAAAG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 880-900 bp 1.0 85.1
rpl32-trnL(uag)_p5 TCCACCGGACAGTCAAAAAG GAGCAGCGTGTCTACCAATT 50.0 50.0 58.0 58.3 875-895 bp 1.0 84.6