Hotspot

ndhF-rpl32

SSC · 114341-115376 · 1036 bp

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Pi 0.036
MarkerSeek score 68.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1036
variable_sites
53
pi
0.036
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.78
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAGTGGAAAAAGGGGTA TTTGACATAGAAGTACGTTTTT 50.0 27.3 58.2 52.0 904-1122 bp 1.0 48.3
ndhF-rpl32_p2 TGGGAGTGGAAAAAGGGGTA TTTTGACATAGAAGTACGTTTT 50.0 27.3 58.2 52.0 905-1123 bp 1.0 48.2
ndhF-rpl32_p3 TGGGAGTGGAAAAAGGGGTA TTTTTGACATAGAAGTACGTTT 50.0 27.3 58.2 52.0 906-1124 bp 1.0 48.2
ndhF-rpl32_p4 TGGGAGTGGAAAAAGGGGTA TTTTGACATAGAAGTACGTTTTT 50.0 26.1 58.2 52.9 905-1123 bp 1.0 48.2
ndhF-rpl32_p5 TGGGAGTGGAAAAAGGGGTA TTTTTGACATAGAAGTACGTTTT 50.0 26.1 58.2 52.9 906-1124 bp 1.0 48.2