Hotspot

rps8-rpl14

LSC · 81587-81819 · 233 bp

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Pi 0.055
MarkerSeek score 73.40
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
233
variable_sites
20
pi
0.055
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.88
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CGGCAATAATGTCCCTACCC GTTAAACCGGGCCGAATACT 55.0 50.0 58.1 58.0 840-876 bp 1.0 85.6
rps8-rpl14_p2 ATAAAACTGCGCCGGATTCT GTTAAACCGGGCCGAATACT 45.0 50.0 58.0 58.0 1458-1494 bp 1.0 83.8
rps8-rpl14_p3 ATAAAACTGCGCCGGATTCT AGAAACACGTATGGGTTCGG 45.0 50.0 58.0 57.9 1510-1546 bp 1.0 83.2
rps8-rpl14_p4 GGGTTGGCTGATACGCTTTA GTTAAACCGGGCCGAATACT 50.0 50.0 58.0 58.0 1074-1100 bp 0.75 73.4
rps8-rpl14_p5 GGGTTGGCTGATACGCTTTA AGAAACACGTATGGGTTCGG 50.0 50.0 58.0 57.9 1126-1152 bp 0.75 72.8