Hotspot

psbB-psbT

LSC · 74458-74981 · 524 bp

Back to Stelis

Pi 0.0375
MarkerSeek score 72.10
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
524
variable_sites
33
pi
0.0375
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 CGGGCTACTTTGAAATCCGA CCCTAGTCGCCATCTCCATA 50.0 55.0 58.0 58.1 1028-1060 bp 1.0 86.3
psbB-psbT_p2 CGGGCTACTTTGAAATCCGA GAGCCATTGCAACTCCCATA 50.0 50.0 58.0 57.9 1288-1329 bp 1.0 86.0
psbB-psbT_p3 GGGGATGGAATAGCTGTTGG CCCTAGTCGCCATCTCCATA 55.0 55.0 58.0 58.1 1331-1363 bp 1.0 85.8
psbB-psbT_p4 TATGGTGGCGAACTCAATGG GAGCCATTGCAACTCCCATA 50.0 50.0 58.0 57.9 1384-1425 bp 1.0 85.8
psbB-psbT_p5 GGGGATGGAATAGCTGTTGG GAGCCATTGCAACTCCCATA 55.0 50.0 58.0 57.9 1591-1632 bp 1.0 85.7