Hotspot

clpP-psbB

LSC · 72128-72930 · 803 bp

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Pi 0.0253
MarkerSeek score 73.70
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
803
variable_sites
36
pi
0.0253
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 894-1031 bp 1.0 86.0
clpP-psbB_p2 TATACGTCAACCCAAGCTGC GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 889-1026 bp 1.0 85.9
clpP-psbB_p3 CTTTTGGAACACCAATGGGC GGCCCAACCAGAAACTAGAG 50.0 55.0 57.8 57.9 840-977 bp 1.0 84.9
clpP-psbB_p4 TTTGGAACACCAATGGGCAT GGCCCAACCAGAAACTAGAG 45.0 55.0 58.3 57.9 838-975 bp 1.0 84.0
clpP-psbB_p5 TCCAGGACTCCGAAAAGGTA GGCCCAACCAGAAACTAGAG 50.0 55.0 57.7 57.9 860-997 bp 1.0 83.6