Hotspot

rbcL-accD

LSC · 55806-56634 · 829 bp

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Pi 0.0231
MarkerSeek score 60.30
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
829
variable_sites
34
pi
0.0231
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AATGAGGGACGTGATCTTGC TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1106-1145 bp 1.0 83.5
rbcL-accD_p2 ACTACAGTTCGGTGGAGGAA TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1203-1242 bp 1.0 83.3
rbcL-accD_p3 GGCTTCAGGGGGTATTCATG TTCTTCACTCCCACCGGTAT 55.0 50.0 58.0 58.1 1269-1308 bp 1.0 83.1
rbcL-accD_p4 GAGCGGTATTTGCCAGAGAA TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1642-1681 bp 1.0 82.4
rbcL-accD_p5 AAGAGCGGTATTTGCCAGAG TTCTTCACTCCCACCGGTAT 50.0 50.0 58.0 58.1 1644-1683 bp 1.0 82.3