Hotspot

trnG(ucc)-trnR(ucu)

LSC · 9520-9670 · 151 bp

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Pi 0.0475
MarkerSeek score 73.20
Primer available yes
Label trnG(ucc)-trnR(ucu)

Feature Metadata

label_name
trnG(ucc)-trnR(ucu)
feature_type
igs
region
LSC
length_bp
151
variable_sites
12
pi
0.0475
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG(ucc)-trnR(ucu)_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 231-235 bp 1.0 67.5
trnG(ucc)-trnR(ucu)_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 234-238 bp 1.0 67.2
trnG(ucc)-trnR(ucu)_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 237-241 bp 1.0 66.7
trnG(ucc)-trnR(ucu)_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 240-244 bp 1.0 66.4
trnG(ucc)-trnR(ucu)_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 235-239 bp 1.0 65.0