Hotspot

rrn5

IRa · 132256-132375 · 120 bp

Back to Stegnosperma

Pi 0.075
MarkerSeek score 69.40
Primer available yes
Label rrn5

Feature Metadata

label_name
rrn5
feature_type
rRNA
region
IRa
length_bp
120
variable_sites
9
pi
0.075
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5_p1 ACCTGGGGCTGTAGTATGTT AATGGTGCATTCCAAGGTGT 50.0 45.0 58.0 58.0 984 bp 0.5 59.6
rrn5_p2 TGGGGCTGTAGTATGTTCCA AATGGTGCATTCCAAGGTGT 50.0 45.0 58.0 58.0 981 bp 0.5 59.6
rrn5_p3 GTCAAGTGGAAGTGCAGTGA AATGGTGCATTCCAAGGTGT 50.0 45.0 58.0 58.0 571 bp 0.5 59.5
rrn5_p4 CAGCTGAGGATGCCTAACAG AATGGTGCATTCCAAGGTGT 55.0 45.0 58.0 58.0 545 bp 0.5 59.5
rrn5_p5 GCTGAGGATGCCTAACAGAC AATGGTGCATTCCAAGGTGT 55.0 45.0 58.1 58.0 543 bp 0.5 59.5
rrn5_p1 AATGGTGCATTCCAAGGTGT ACCTGGGGCTGTAGTATGTT 45.0 50.0 58.0 58.0 984 bp 0.5 59.6
rrn5_p2 AATGGTGCATTCCAAGGTGT TGGGGCTGTAGTATGTTCCA 45.0 50.0 58.0 58.0 981 bp 0.5 59.6
rrn5_p3 AATGGTGCATTCCAAGGTGT GTCAAGTGGAAGTGCAGTGA 45.0 50.0 58.0 58.0 571 bp 0.5 59.5
rrn5_p4 AATGGTGCATTCCAAGGTGT CAGCTGAGGATGCCTAACAG 45.0 55.0 58.0 58.0 545 bp 0.5 59.5
rrn5_p5 AATGGTGCATTCCAAGGTGT GCTGAGGATGCCTAACAGAC 45.0 55.0 58.0 58.1 543 bp 0.5 59.5