Hotspot

trnR-ACG-rrn5

IRa · 132000-132255 · 256 bp

Back to Stegnosperma

Pi 0.0898

MarkerSeek score 71.50

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: IRa
length_bp: 256
variable_sites: 23
pi: 0.0898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	CCACTTCGACAAAAGACCCA	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	877-880 bp	1.0	84.4
trnR-ACG-rrn5_p2	ACTTGCTCTGCTATTCTGCC	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	1023-1026 bp	1.0	83.8
trnR-ACG-rrn5_p3	ACTTGCTCTGCTATTCTGCC	TGGTGGTCAAACTGTACTGC	50.0	50.0	58.0	58.0	656 bp	0.5	59.4
trnR-ACG-rrn5_p4	CCACTTCGACAAAAGACCCA	TGGTGGTCAAACTGTACTGC	50.0	50.0	58.0	58.0	510 bp	0.5	59.4
trnR-ACG-rrn5_p5	ACTTGCTCTGCTATTCTGCC	CAGCTGAGGATGCCTAACAG	50.0	55.0	58.0	58.0	997 bp	0.5	59.3