Hotspot

rps15-ycf1

SSC · 124923-125215 · 293 bp

Back to Stegnosperma

Pi 0.0444
MarkerSeek score 78.00
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
293
variable_sites
13
pi
0.0444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CAGACCCCCTGTTTTCTTCT AGCTTGTATGAATCGTTATTGGT 50.0 34.8 57.0 56.3 454-455 bp 1.0 42.3
rps15-ycf1_p2 CAGACCCCCTGTTTTCTTCTT AGCTTGTATGAATCGTTATTGGT 47.6 34.8 57.8 56.3 454-455 bp 1.0 42.3
rps15-ycf1_p3 CAGACCCCCTGTTTTCTTCT AGCTTGTATGAATCGTTATTGGTT 50.0 33.3 57.0 57.0 454-455 bp 1.0 42.3
rps15-ycf1_p4 CAGACCCCCTGTTTTCTTCT GCTTGTATGAATCGTTATTGGT 50.0 36.4 57.0 54.9 453-454 bp 1.0 42.3
rps15-ycf1_p5 TGAAATTCAACAGACCCCCT AGCTTGTATGAATCGTTATTGGT 45.0 34.8 56.6 56.3 464-465 bp 1.0 42.2