Hotspot

rps16-trnQ-UUG

LSC · 5916-6624 · 709 bp

Back to Stegnosperma

Pi 0.0183

MarkerSeek score 63.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 12
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTTCTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	744-789 bp	1.0	84.6
rps16-trnQ-UUG_p2	GTTCTTCAAGTCGCACGTTG	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.0	58.2	774-819 bp	1.0	83.8
rps16-trnQ-UUG_p3	ATGTTCTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	746-791 bp	1.0	83.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	737-782 bp	1.0	83.5
rps16-trnQ-UUG_p5	CATGTTCTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	747-792 bp	1.0	82.9