Hotspot

ycf2

IRb · 88768-95658 · 6891 bp

Back to Stauntonia

Pi 0.0216

MarkerSeek score 52.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6891
variable_sites: 223
pi: 0.0216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CTTTGACCCCCGAATTTGGA	CGATTTCTTCCGGAAGCAGA	50.0	50.0	58.1	58.0	1286 bp	0.666667	65.9
ycf2_p2	CTTTGACCCCCGAATTTGGA	TCGATTTCTTCCGGAAGCAG	50.0	50.0	58.1	58.0	1287 bp	0.666667	65.9
ycf2_p3	TCTTTGACCCCCGAATTTGG	CGATTTCTTCCGGAAGCAGA	50.0	50.0	58.1	58.0	1287 bp	0.666667	65.9
ycf2_p4	TTTGACCCCCGAATTTGGAG	CGATTTCTTCCGGAAGCAGA	50.0	50.0	58.1	58.0	1285 bp	0.666667	65.9
ycf2_p5	TTTGACCCCCGAATTTGGAG	TCGATTTCTTCCGGAAGCAG	50.0	50.0	58.1	58.0	1286 bp	0.666667	65.9