Pi
0.032
MarkerSeek score
62.90
Primer available
yes
Label
rrn23
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rrn23_p1 | ACCTTAGGATGGCAGCCTTA | AAACTTCTGTCGCTCCATCC | 50.0 | 50.0 | 58.1 | 57.9 | 2933-2935 bp | 1.0 | 82.1 |
| rrn23_p2 | ACCTTAGGATGGCAGCCTTA | GGGGAAGTCGGAATAGGAGA | 50.0 | 55.0 | 58.1 | 57.9 | 2864 bp | 0.666667 | 65.2 |
| rrn23_p3 | GTTCGAGTCCGCTTATCTCC | GGGGAAGTCGGAATAGGAGA | 55.0 | 55.0 | 57.9 | 57.9 | 3000 bp | 0.666667 | 65.1 |
| rrn23_p4 | GGATGGCAGCCTTAAAGTGA | GGGGAAGTCGGAATAGGAGA | 50.0 | 55.0 | 57.9 | 57.9 | 2858 bp | 0.666667 | 65.0 |
| rrn23_p5 | TCCTTCCATTTAGCAGCACC | GGGGAAGTCGGAATAGGAGA | 50.0 | 55.0 | 57.9 | 57.9 | 2881 bp | 0.666667 | 65.0 |
| rrn23_p1 | GCTCTGGGGAAGTCGGAAT | ACCTTAGGATGGCAGCCTTA | 57.9 | 50.0 | 59.1 | 58.1 | 2869-2870 bp | 1.0 | 66.2 |
| rrn23_p2 | GCTCTGGGGAAGTCGGAAT | GGATGGCAGCCTTAAAGTGA | 57.9 | 50.0 | 59.1 | 57.9 | 2863-2864 bp | 1.0 | 66.0 |
| rrn23_p3 | GCTCTGGGGAAGTCGGAAT | AGGATGGCAGCCTTAAAGTG | 57.9 | 50.0 | 59.1 | 57.9 | 2864-2865 bp | 1.0 | 66.0 |
| rrn23_p4 | GCTCTGGGGAAGTCGGAA | ACCTTAGGATGGCAGCCTTA | 61.1 | 50.0 | 58.6 | 58.1 | 2869-2870 bp | 1.0 | 62.0 |
| rrn23_p5 | GCTCTGGGGAAGTCGGAA | GGATGGCAGCCTTAAAGTGA | 61.1 | 50.0 | 58.6 | 57.9 | 2863-2864 bp | 1.0 | 61.9 |