Hotspot

rrn16

IRb · 103047-104537 · 1491 bp

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Pi 0.0349

MarkerSeek score 54.60

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRb
length_bp: 1491
variable_sites: 78
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	GGCTCCAAGTTGTTCAGGAA	CAGACGTAGCTCGCTTCTTT	50.0	50.0	58.0	58.0	1948-1962 bp	1.0	83.6
rrn16_p2	GGCTCCAAGTTGTTCAGGAA	TCGCTTCTTTTTGGGTGTGA	50.0	45.0	58.0	58.0	1938-1952 bp	1.0	83.4
rrn16_p3	GGCTCCAAGTTGTTCAGGAA	TGAATCGCCCCATCTATCCT	50.0	50.0	58.0	58.0	2378-2396 bp	1.0	83.3
rrn16_p4	TAGTGGCGTTGAGTTTCTCG	CAGACGTAGCTCGCTTCTTT	50.0	50.0	57.9	58.0	1928-1934 bp	1.0	83.3
rrn16_p5	TAGTGGCGTTGAGTTTCTCG	TGAATCGCCCCATCTATCCT	50.0	50.0	57.9	58.0	2358-2368 bp	1.0	83.0