Hotspot

ycf2-trnI-CAU

IRa · 155598-155665 · 68 bp

Back to Stauntonia

Pi 0.0588

MarkerSeek score 63.90

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 68
variable_sites: 6
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	CCAAATTCGGGGGTCAAAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	285-289 bp	1.0	85.4
ycf2-trnI-CAU_p2	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	812 bp	0.666667	66.4
ycf2-trnI-CAU_p3	AATCATGGTCCTTGCGGATC	CCACAATGACGCTGTCCATA	50.0	50.0	58.0	58.0	1435 bp	0.666667	66.3
ycf2-trnI-CAU_p4	CTCCAAATTCGGGGGTCAAA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	291 bp	0.666667	65.9
ycf2-trnI-CAU_p5	TCCAAATTCGGGGGTCAAAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	290 bp	0.666667	65.9