Hotspot

rpl32-trnL-UAG

SSC · 116300-117285 · 986 bp

Back to Stauntonia

Pi 0.00349

MarkerSeek score 63.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 986
variable_sites: 5
pi: 0.00349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGACAGCGGTAAAAGCTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1083-1114 bp	1.0	82.4
rpl32-trnL-UAG_p2	TTGGACAGCGGTAAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1084-1115 bp	1.0	82.4
rpl32-trnL-UAG_p3	TGGACAGCGGTAAAAGCTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1084-1115 bp	1.0	82.3
rpl32-trnL-UAG_p4	TTGGACAGCGGTAAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1085-1116 bp	1.0	82.3
rpl32-trnL-UAG_p5	TGGACAGCGGTAAAAGCTTT	TTTAGAAGATGCCGCCACTC	45.0	50.0	58.0	58.0	1143-1174 bp	1.0	82.1