Hotspot

rps12-trnV-GAC

IRb · 100851-102747 · 1897 bp

Back to Stauntonia

Pi 0.019

MarkerSeek score 44.90

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1897
variable_sites: 54
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TACTCCGACAGCATCTAGGG	CCTCAGCCTACGGGGTATTA	55.0	55.0	58.0	58.0	2566-2584 bp	1.0	82.8
rps12-trnV-GAC_p2	AGCGATTCACATGGCATCAT	CCTCAGCCTACGGGGTATTA	45.0	55.0	58.0	58.0	2648-2667 bp	1.0	82.7
rps12-trnV-GAC_p3	TCTACAACGCACTAGAACGC	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	2602-2621 bp	1.0	82.7
rps12-trnV-GAC_p4	TACTCCGACAGCATCTAGGG	CATTGCGGAAAATTCCCCAC	55.0	50.0	58.0	58.0	2750-2758 bp	0.666667	66.5
rps12-trnV-GAC_p5	AGCGATTCACATGGCATCAT	CATTGCGGAAAATTCCCCAC	45.0	50.0	58.0	58.0	2833-2841 bp	0.666667	66.4