Hotspot

trnI-CAU-ycf2

IRb · 88700-88767 · 68 bp

Back to Stauntonia

Pi 0.0882
MarkerSeek score 64.80
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
68
variable_sites
9
pi
0.0882
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC TGTCGTGTCATTGATTTCTGC 50.0 42.9 58.0 57.2 126 bp 1.0 71.1
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC GTCGTGTCATTGATTTCTGCT 50.0 42.9 58.0 56.9 125 bp 1.0 69.0
trnI-CAU-ycf2_p3 AGTTGGGCGCTTTAACCATT TGTCGTGTCATTGATTTCTGC 45.0 42.9 58.4 57.2 127 bp 1.0 68.2
trnI-CAU-ycf2_p4 TTGGGCGCTTTAACCATTCA TGTCGTGTCATTGATTTCTGC 45.0 42.9 58.4 57.2 125 bp 1.0 68.2
trnI-CAU-ycf2_p5 GTTGGGCGCTTTAACCATTC ATGTCGTGTCATTGATTTCTGC 50.0 40.9 58.0 57.7 127 bp 1.0 66.8