Hotspot

accD-psaI

LSC · 60555-61241 · 687 bp

Back to Stauntonia

Pi 0.0248

MarkerSeek score 63.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 24
pi: 0.0248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTACACGGTTTCTTTCCC	GCCATTGAAATTGCCGGAAA	50.0	45.0	57.8	57.9	802-850 bp	1.0	80.4
accD-psaI_p2	GCTACACGGTTTCTTTCCCT	GCCATTGAAATTGCCGGAAA	50.0	45.0	57.8	57.9	801-849 bp	1.0	80.4
accD-psaI_p3	AGCTACACGGTTTCTTTCCC	GAAGCCATTGAAATTGCCGG	50.0	50.0	57.8	58.4	805-853 bp	1.0	78.3
accD-psaI_p4	GCTACACGGTTTCTTTCCCT	GAAGCCATTGAAATTGCCGG	50.0	50.0	57.8	58.4	804-852 bp	1.0	78.3
accD-psaI_p5	GCTACACGGTTTCTTTCCCT	GCCGGAAATACTAGGCCTAC	50.0	55.0	57.8	57.5	789-837 bp	1.0	77.6