Hotspot

ndhF

SSC · 115588-117816 · 2229 bp

Back to Staphylea

Pi 0.0015

MarkerSeek score 43.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 6
pi: 0.0015
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2411-2468 bp	1.0	81.2
ndhF_p2	GCAGAATACCGTCACCCATT	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2535-2592 bp	1.0	81.1
ndhF_p3	GCAGAATACCGTCACCCATT	ATCCGCCCTCTATCGATTGA	50.0	50.0	58.0	58.1	2860-2866 bp	0.5	59.3
ndhF_p4	ACAGGAACAAGAGGGATCCA	ATCCGCCCTCTATCGATTGA	50.0	50.0	58.0	58.1	2736-2742 bp	0.5	59.3
ndhF_p5	AAGAGGGATCCACCGAAGAA	ATCCGCCCTCTATCGATTGA	50.0	50.0	58.0	58.1	2728-2734 bp	0.5	59.3