Hotspot

ndhF-rpl32

SSC · 117817-118685 · 869 bp

Back to Staphylea

Pi 0.0115

MarkerSeek score 57.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 869
variable_sites: 18
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACGAAAGGT	ACCCCCTCCTTTTCCAAATA	50.0	45.0	57.7	56.0	991-1000 bp	1.0	63.5
ndhF-rpl32_p2	CTGGAAGTGGAACGAAAGGT	AATACCCCCTCCTTTTCCAA	50.0	45.0	57.7	56.0	994-1003 bp	1.0	63.4
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	ATACCCCCTCCTTTTCCAAA	50.0	45.0	57.7	56.0	993-1002 bp	1.0	63.4
ndhF-rpl32_p4	CTGGAAGTGGAACGAAAGGT	TACCCCCTCCTTTTCCAAAT	50.0	45.0	57.7	56.0	992-1001 bp	1.0	63.4
ndhF-rpl32_p5	AAAGTAGAAGTCCCACCCCT	ACCCCCTCCTTTTCCAAATA	50.0	45.0	57.6	56.0	1024-1033 bp	1.0	62.0