Hotspot

rpl33-rps18

LSC · 72646-72929 · 284 bp

Back to Staphylea

Pi 0.0088

MarkerSeek score 63.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 4
pi: 0.0088
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGGTTCACGGGGAGATAAAG	TAATCAATTCGATCCCCCGG	55.0	50.0	58.1	57.1	386-399 bp	1.0	76.1
rpl33-rps18_p2	CGGTTCACGGGGAGATAAAG	GCAAACGCCTACGAAAAGAT	55.0	45.0	58.1	57.1	355-368 bp	1.0	76.1
rpl33-rps18_p3	CGGTTCACGGGGAGATAAAG	GGCAAACGCCTACGAAAAGA	55.0	50.0	58.1	59.1	356-369 bp	1.0	74.2
rpl33-rps18_p4	CGGTTCACGGGGAGATAAAG	AATCAATTCGATCCCCCGGT	55.0	50.0	58.1	59.2	385-398 bp	1.0	73.7
rpl33-rps18_p5	CGGTTCACGGGGAGATAAAG	ATCAATTCGATCCCCCGGTT	55.0	50.0	58.1	59.2	384-397 bp	1.0	73.7