Pi
0.00614
MarkerSeek score
57.00
Primer available
yes
Label
trnT-UGU-trnL
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL_p1 | CAAATAGAGAAAGGCCGGCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 1154-1166 bp | 1.0 | 81.3 |
| trnT-UGU-trnL_p2 | CAAATAGAGAAAGGCCGGCT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.0 | 58.1 | 1162-1174 bp | 1.0 | 80.7 |
| trnT-UGU-trnL_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1099-1111 bp | 1.0 | 80.6 |
| trnT-UGU-trnL_p4 | GACCGTCGCATTACAAATGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 1117-1129 bp | 1.0 | 80.2 |
| trnT-UGU-trnL_p5 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1107-1119 bp | 1.0 | 80.1 |