Hotspot

psaA-ycf3

LSC · 45182-45948 · 767 bp

Back to Staphylea

Pi 0.00914

MarkerSeek score 66.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 13
pi: 0.00914
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCCGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	957-964 bp	1.0	80.7
psaA-ycf3_p2	TGAAAAATGACCCGGTCTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	939-946 bp	1.0	80.7
psaA-ycf3_p3	CTGGCCCATTCTTCGAAAGA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	941-948 bp	1.0	80.5
psaA-ycf3_p4	TCTGGCCCATTCTTCGAAAG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	942-949 bp	1.0	80.5
psaA-ycf3_p5	CTGGCCCATTCTTCGAAAGA	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	923-930 bp	1.0	80.5