Hotspot

psbM-trnD

LSC · 31896-33054 · 1159 bp

Back to Staphylea

Pi 0.00375

MarkerSeek score 62.50

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 8
pi: 0.00375
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	1140-1267 bp	1.0	78.6
psbM-trnD_p2	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1139-1266 bp	1.0	77.7
psbM-trnD_p3	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	1141-1268 bp	1.0	76.9
psbM-trnD_p4	AGAATGAACAGCGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.6	58.5	1143-1270 bp	1.0	75.6
psbM-trnD_p5	ACTAGAATGAACAGCGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	1146-1273 bp	1.0	74.7