Hotspot

trnL-UAG-rpl32

SSC · 123147-123547 · 401 bp

Back to Staehelina

Pi 0.015

MarkerSeek score 59.10

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 9
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	50.0	50.0	58.0	57.6	533-999 bp	1.0	78.1
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	50.0	50.0	58.0	57.6	534-1000 bp	1.0	78.1
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	516-982 bp	1.0	77.9
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	517-983 bp	1.0	77.9
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	AAGGAAAGGATATTGGGCGG	50.0	50.0	57.9	57.6	543-1009 bp	1.0	77.1