Hotspot

trnC-GCA-petN

LSC · 9329-10168 · 840 bp

Back to Staehelina

Pi 0.0137

MarkerSeek score 69.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 17
pi: 0.0137
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CTAAAGCCCACTTCTTCCCC	50.0	55.0	58.1	57.9	938-959 bp	1.0	81.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	875-896 bp	1.0	81.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AAGCCCACTTCTTCCCCATA	50.0	50.0	58.1	58.3	935-956 bp	1.0	80.2
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	893-914 bp	1.0	79.5
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CTAAAGCCCACTTCTTCCCC	50.0	55.0	58.4	57.9	956-977 bp	1.0	79.4