Hotspot

ccsA

SSC · 122203-123162 · 960 bp

Back to Stachyurus

Pi 0

MarkerSeek score 32.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CAGGGACCTTCACACTTCAC	TGATTCAACGGGTTCTCGAC	55.0	50.0	58.1	57.9	1095-1097 bp	1.0	78.5
ccsA_p2	GGCGGCATAGCATCTTCTAA	TGATTCAACGGGTTCTCGAC	50.0	50.0	57.8	57.9	1178-1180 bp	1.0	78.0
ccsA_p3	GACAGGGACCTTCACACTTC	TGATTCAACGGGTTCTCGAC	55.0	50.0	57.8	57.9	1097-1099 bp	1.0	78.0
ccsA_p4	AATTGAGGGACAGGGACCTT	TGATTCAACGGGTTCTCGAC	50.0	50.0	58.2	57.9	1105-1107 bp	1.0	77.5
ccsA_p5	CAGGGACCTTCACACTTCAC	TACGCTGCCCCATTTTTGTA	55.0	45.0	58.1	57.8	1049-1051 bp	1.0	77.5