Hotspot

rps15-ycf1

SSC · 131409-131803 · 395 bp

Back to Stachyurus

Pi 0.00624

MarkerSeek score 40.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 4
pi: 0.00624
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCAAAATAACTGAGATGAATGCA	ATGGCGGTCGCTTTAGTATG	33.3	50.0	57.7	58.1	487-489 bp	1.0	45.9
rps15-ycf1_p2	TGCAAAATAACTGAGATGAATGCA	GGCGGTCGCTTTAGTATGAT	33.3	50.0	57.7	57.8	485-487 bp	1.0	45.8
rps15-ycf1_p3	GCAAAATAACTGAGATGAATGCAC	ATGGCGGTCGCTTTAGTATG	37.5	50.0	57.4	58.1	486-488 bp	1.0	43.6
rps15-ycf1_p4	GCAAAATAACTGAGATGAATGCAC	GGCGGTCGCTTTAGTATGAT	37.5	50.0	57.4	57.8	484-486 bp	1.0	43.4
rps15-ycf1_p5	TGCAAAATAACTGAGATGAATGCA	AATGGCGGTCGCTTTAGTAT	33.3	45.0	57.7	57.4	488-490 bp	1.0	42.1